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up vote 3 down vote favorite We have an old BioRad ICycler Thermal Cycler with MyIQ single color fluorescent detector. Absorption ratios and for RNA. up vote 11 down vote favorite. an I want to perform reverse transcription and then PCR. To get good results, in which range should the absorption ratios nm and nm be?
And what is the idea behind measuring those ratios? Rolling a D10 (09) for hitpoints. What do I get with a Sample Reports S34 Copyright Houghton Mifflin Company.
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